NDUFA7 Antibody (OTI2G4)

Novus Biologicals | Catalog # NBP2-00564

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G4
View all NDUFA7 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NDUFA7 (NP_004992) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFA7 Antibody (OTI2G4)

Western Blot: NDUFA7 Antibody (2G4) [NBP2-00564] Analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFA7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Immunocytochemistry/ Immunofluorescence: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Immunocytochemistry/Immunofluorescence: NDUFA7 Antibody (2G4) [NBP2-00564] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDUFA7.
Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (2G4) [NBP2-00564] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDUFA7 antibody, and then analyzed by flow cytometry.
Western Blot: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Western Blot: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Western Blot: NDUFA7 Antibody (2G4) [NBP2-00564] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFA7.
Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (2G4) [NBP2-00564] - Analysis of Hela cells, using anti-NDUFA7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (OTI2G4) [NBP2-00564]

Flow Cytometry: NDUFA7 Antibody (2G4) [NBP2-00564] - Analysis of Jurkat cells, using anti-NDUFA7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for NDUFA7 Antibody (OTI2G4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.62 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFA7

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

B14.5a, CI-B14.5a, complex I B14.5a subunit, Complex I-B14.5a, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NADH-ubiquinone oxidoreductase subunit B14.5a

Entrez Gene IDs

4701 (Human)

Gene Symbol

NDUFA7

Additional NDUFA7 Products

Product Documents for NDUFA7 Antibody (OTI2G4)

Certificate of Analysis

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Product Specific Notices for NDUFA7 Antibody (OTI2G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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