NDUFAF3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-48638
![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0012.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSF
Reactivity Notes
Mouse (82%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NDUFAF3 Antibody - BSA Free
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0002.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human testis shows strong cytoplasmic positivity in with a granular pattern in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0004.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human testis.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0005.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human kidney.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0006.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human colon.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0007.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human urinary bladder.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0008.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human fallopian tube, kidney, prostate and testis using Anti-NDUFAF3 antibody NBP2-48638 (A) shows similar protein distribution across tissues to independent antibody NBP1-89382 (B).![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0009.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0010.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.![Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]](https://resources.rndsystems.com/images/products/NDUFAF3-Antibody-Immunohistochemistry-Paraffin-NBP2-48638-img0011.jpg "Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]")
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638]
Immunohistochemistry-Paraffin: NDUFAF3 Antibody [NBP2-48638] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.Applications for NDUFAF3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NDUFAF3
Alternate Names
C3orf60, chromosome 3 open reading frame 60, DKFZp564J0123, DKFZP564J0123,2P1, E3-3, MGC10527, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3, nuclear protein E3-3
Gene Symbol
NDUFAF3
Additional NDUFAF3 Products
Product Documents for NDUFAF3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NDUFAF3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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