NEK2 Antibody (2F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00004751-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F6

Format

Azide and BSA Free
View all NEK2 Primary Antibodies »

Product Specifications

Immunogen

NEK2 (AAH43502, 331 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR

Specificity

NEK2 (2F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NEK2 Antibody (2F6) - Azide and BSA Free

Western Blot: NEK2 Antibody (2F6) [H00004751-M01]

Western Blot: NEK2 Antibody (2F6) [H00004751-M01]

Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01]

Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01]

Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01]

Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01]

Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 transfected lysate using anti-NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: NEK2 Antibody (2F6) [H00004751-M01] - Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.

Applications for NEK2 Antibody (2F6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:2000

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEK2

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Entrez Gene IDs

4751 (Human)

Gene Symbol

NEK2

OMIM

604043 (Human)

UniProt

AAH43502 (Human)

Additional NEK2 Products

Product Documents for NEK2 Antibody (2F6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NEK2 Antibody (2F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for NEK2 Antibody (2F6) - Azide and BSA Free

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Protocols

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FAQs

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