Neuropeptide Y Antibody (8) - BSA Free
Novus Biologicals | Catalog # NBP1-05166
![Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]](https://resources.rndsystems.com/images/products/Neuropeptide-Y-Antibody-8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05166-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Functional Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8
Format
BSA Free
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Product Specifications
Immunogen
Synthetic human/rat neuropeptide Y
Epitope
The epitope of this antibody is probably around the mid-molecular region, NPY (18-24).
Specificity
Binds full length human/rat NPY. Cross-reactivity with NPY (1-24), NPY (18-36), PYY or PP was <0.2%.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Neuropeptide Y Antibody (8) - BSA Free
Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]
Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]](https://resources.rndsystems.com/images/products/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0005.jpg "Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]")
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (interneurons in the cortex) diluted 1/1000![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]](https://resources.rndsystems.com/images/products/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0003.jpg "Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]")
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]](https://resources.rndsystems.com/images/products/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0004.jpg "Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]")
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166]
Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000Applications for Neuropeptide Y Antibody (8) - BSA Free
Application
Recommended Usage
ELISA
1:16000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Application Notes
ELISA: NBP1-05166 binds free NPY in solution and NBP1-05166 reacts in ELISA directly with NPY coated directly onto the microtiter well.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Neuropeptide Y/NPY
Alternate Names
NPY, PYY4
Gene Symbol
NPY
Additional Neuropeptide Y/NPY Products
Product Documents for Neuropeptide Y Antibody (8) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Neuropeptide Y Antibody (8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Neuropeptide Y Antibody (8) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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