NLK Antibody (6G5V10)

Name: NLK Antibody (6G5V10)
Brand: Novus Biologicals
SKU: NBP3-15728
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15728

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6G5V10 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human NLK (NP_057315.3). AVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NLK Antibody (6G5V10)

Western Blot: NLK Antibody (6G5V10) [NBP3-15728]

Western Blot: NLK Antibody (6G5V10) [NBP3-15728] - Analysis of extracts of various cell lines, using NLK antibody (NBP3-15728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728]

Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728] - Human placenta using NLK Rabbit mAb (NBP3-15728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for NLK Antibody (6G5V10)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NLK

Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3

Alternate Names

DKFZp761G1211, EC 2.7.11, EC 2.7.11.24, FLJ21033, LAK1, nemo like kinase, nemo-like kinaseserine/threonine-protein kinase NLK, Protein LAK1

Gene Symbol

NLK

Additional NLK Products

Product Documents for NLK Antibody (6G5V10)

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Certificate of Analysis

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Product Specific Notices for NLK Antibody (6G5V10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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