NM23-H1 Antibody (23GB2195)

Novus Biologicals | Catalog # NBP3-51024

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2195
View all NM23-H1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human NM23-H1 (Uniprot: P15531)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NM23-H1 Antibody (23GB2195)

NM23-H1 Antibody (23GB2195)

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

NM23-H1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: NME1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;NME1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
NM23-H1 Antibody (23GB2195)

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

Immunocytochemical staining of HepG2 cells with NM23-H1 antibody (1:1,000). Nuclei were stained blue with DAPI; NM23-H1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
NM23-H1 Antibody (23GB2195)

Flow Cytometry: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

Validation of NM23-H1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NM23-H1 antibody (1:2,000) and analyzed using BD flow cytometer.
NM23-H1 Antibody (23GB2195)

Flow Cytometry: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

Flow cytometric analysis of NM23-H1expression in HepG2 cells using NM23-H1 antibody (1:2,000). Green, isotype control; red, NME1.
NM23-H1 Antibody (23GB2195)

Western Blot: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

Western blotting analysis using NM23-H1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
NM23-H1 Antibody (23GB2195)

Western Blot: NM23-H1 Antibody (23GB2195)[NBP3-51024] -

Western blotting analysis using NM23-H1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NM23-H1 Antibody (23GB2195)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NM23-H1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody (23GB2195)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NM23-H1 Antibody (23GB2195)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for NM23-H1 Antibody (23GB2195)

There are currently no reviews for this product. Be the first to review NM23-H1 Antibody (23GB2195) and earn rewards!

Have you used NM23-H1 Antibody (23GB2195)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies