NM23-H1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30884

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Canine (93%), Primate (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NM23-H1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NM23-H1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (93%) and Chimpanzee (93%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit NM23-H1 Antibody - BSA Free (NBP1-30884) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NM23-H1 Antibody - BSA Free

Western Blot: NM23-H1 Antibody [NBP1-30884]

Western Blot: NM23-H1 Antibody [NBP1-30884]

Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody [NBP1-30884]

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody [NBP1-30884]

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP1-30884] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
NM23-H1 Antibody

Immunohistochemistry: NM23-H1 Antibody [NBP1-30884] -

Immunohistochemistry: NM23-H1 Antibody [NBP1-30884] - NM23-H1 antibody [C1C3] detects NM23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
NM23-H1 antibody [C1C3] (NBP1-30884) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
NM23-H1 Antibody

Western Blot: NM23-H1 Antibody [NBP1-30884] -

Western Blot: NM23-H1 Antibody [NBP1-30884] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NM23-H1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NM23-H1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NM23-H1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

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Protocols

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