non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13579

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT144

Format

Azide and BSA Free
View all Non-muscle Myosin IIA Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1943 of human non-muscle Myosin IIA. The exact sequence is proprietary.

Modification

p Ser1943

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

227 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human mammary gland. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:20000.
Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000.
Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human colon. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] -

MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH9 (phospho Ser1943) protein stained by MYH9 (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Non-muscle Myosin IIA

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Gene Symbol

MYH9

Additional Non-muscle Myosin IIA Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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