Noxa Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56368

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Noxa Primary Antibodies »

Product Specifications

Immunogen

This Noxa antibody was developed by immunizing rabbits with a mixture of synthetic peptides containing amino acids 51-66 and 75-90 of mouse Noxa.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: amino acids 51-66: Rat (77%); amino acids 75-90: Rat (93%), Human (76%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Noxa Antibody - BSA Free

Western Blot: Noxa Antibody [NB100-56368]

Western Blot: Noxa Antibody [NB100-56368]

Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368]

Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368]

Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Immunocytochemistry/ Immunofluorescence: Noxa Antibody [NB100-56368]

Immunocytochemistry/ Immunofluorescence: Noxa Antibody [NB100-56368]

Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Noxa Antibody [NB100-56368]

Western Blot: Noxa Antibody [NB100-56368]

Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.

Applications for Noxa Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:50 - 1:250

Immunohistochemistry-Paraffin

1:50 - 1:250. Use reported in scientific literature (Inta et al. (2006))

Western Blot

1 - 2 ug/mL

Reviewed Applications

Read 1 review rated 5 using NB100-56368 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Noxa

Noxa, phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, PMAIP1 (Human 6 kDa and Mouse 11.6 kDa) is a Bcl2 homology 3 (BH3) domain containing protein which plays a role in the regulation of apoptosis, specifically a pro-apoptotic role. Human Noxa contains only one BH3 domain, while the mouse and rat proteins contain two BH3 domains. The human protein is encoded by Exon 1 and 3, with Exon 2 present only in two splice variants. Human Noxa splice variants lack a BH3 domain, have no known function, and are rapidly targeted for degradation (1).

Noxa mediated apoptosis may follow its transcriptional activation by p53 as part of the DNA damage response. However, Noxa expression may be induced by HIF-1 alpha under hypoxia, promoting apoptosis independently of p53 (1). Inhibition of the anti-apoptotic Bcl2 family member Myeloid cell leukemia-1 (Mcl-1) by Noxa, leads to the activation of pro-apoptotic Bcl2 homologous antagonist killer (Bak) and Bcl2 associated X (Bax) proteins, and apoptosis through the intrinsic mitochondrial pathway (2). Other BH3 only proteins such as Bim, Puma, Bad and Bid act via the same mechanism, targeting Mcl-1 and inducing Bak/Bax mediated mitochondrial outer membrane permeabilization (MOMP), cytochrome c release and caspase 9 activation (3). Overexpression of antiapoptotic Bcl2 family members is common in various types of cancer including prostate cancer. A recent study identified antiapoptotic Bcl2 proteins involved in the development of resistance towards the androgen receptor antagonist enzalutamide, which is used for the treatment of metastatic castration-resistant prostate cancer (mCRPC) (3).

References

1.Ploner, C., Kofler, R., & Villunger, A. (2008). Noxa: At the tip of the balance between life and death. Oncogene. https://doi.org/10.1038/onc.2009.46

2.Xiang, W., Yang, C. Y., & Bai, L. (2018). MCL-1 inhibition in cancer treatment. OncoTargets and Therapy. https://doi.org/10.2147/OTT.S146228

3.Pilling, A. B., & Hwang, C. (2019). Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. https://doi.org/10.1002/pros.23843

Alternate Names

adult T cell leukemia-derived PMA-responsive, APR, Immediate-early-response protein APR, NOXAPMA-induced protein 1, phorbol-12-myristate-13-acetate-induced protein 1, PMA-Induced Protein 1, Protein Noxa

Entrez Gene IDs

5366 (Human); 58801 (Mouse); 492821 (Rat)

Gene Symbol

PMAIP1

UniProt

Q9JM54 (Mouse)

Additional Noxa Products

Product Documents for Noxa Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Noxa Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Noxa Antibody - BSA Free

Customer Reviews for Noxa Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: whole cell lysate of human colon cancer cell HCT116
    Species: Human
    Verified Customer | Posted 04/04/2014
    Noxa expression was stably reduced in HCT116 cells by shRNA
    Noxa Antibody - BSA Free NB100-56368

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