NOXO1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77899

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NOXO1 Primary Antibodies »

Product Specifications

Immunogen

NOXO1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 238-252 of human NOXO1 protein. (Uniprot: Q8NFA2)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known. Mouse reactivity reported in verified customer review.

Specificity

Anti-NOXO-1 antibody is directed against human NOXO1 protein. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for NOXO1 Antibody - BSA Free

Western Blot: NOXO1 Antibody [NBP1-77899]

Western Blot: NOXO1 Antibody [NBP1-77899]

Western Blot: NOXO1 Antibody [NBP1-77899] - NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown).
Immunohistochemistry: NOXO1 Antibody [NBP1-77899]

Immunohistochemistry: NOXO1 Antibody [NBP1-77899]

Immunohistochemistry: NOXO1 Antibody [NBP1-77899] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of the lamina propia in human colon epithelium and macrophages at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
NOXO1 Antibody

NOXO1 Antibody

Western blot using Affinity Purified anti-NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1. Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, 3T3, untransfected 293 or WT MEF cells. A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Personal Communication, Zhenggang Liu, NIH, CCR, Bethesda, MD.
NOXO1 Antibody - BSA Free

Simple Western: Rabbit Polyclonal NOXO1 Antibody [NBP1-77899]

NOXO1 in mouse lungs. The first lane is a control; the second lane is treated. Image from a verified customer review.

Applications for NOXO1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Simple Western

Validated for Simple Western from a verified customer review.

Western Blot

1:1000-1:10000
Application Notes
This product has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

Reviewed Applications

Read 3 reviews rated 3.3 using NBP1-77899 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NOXO1

NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.

Alternate Names

MGC20258, NADPH oxidase organizer 1, Nox organizer 1, Nox-organizing protein 1, P41NOX, P41NOXA, P41NOXB, P41NOXC, regulatory protein P41NOX, SH3 and PX domain-containing protein 5, SH3PXD5NADPH oxidase regulatory protein, SNX28

Gene Symbol

NOXO1

UniProt

Q8NFA2 (Human)

Additional NOXO1 Products

Product Documents for NOXO1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NOXO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NOXO1 Antibody - BSA Free (3)

3.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
33%

Have you used NOXO1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 的 3 reviews Showing All
Filter By:
Clear Filters X
  • Noxo1 protein expression
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    Noxo1 expression in mouse lung homogenates using Western blot
    I tested the protein expression of noxo1 in mouse lung homogenates after deletion of a NADPH oxidase upon smoke exposure
    NOXO1 Antibody - BSA Free NBP1-77899
  • Noxo1 protein expression
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse, Mouse and Mouse
    Verified Customer | Posted 09/08/2025
    Noxo1 protein expression in mouse lung homogenates
    Noxo1 protein expression in mouse lung homogenates using JESS
    NOXO1 Antibody - BSA Free NBP1-77899
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • NOXO1 in mouse lungs
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 06/03/2025
    NOXO1 in mouse lungs. The first lane is a control, the second lane is treated.
    Antibodies were diluted in antibody diluent 2 in 1:20 concentration. Incubation 60min.
    NOXO1 Antibody - BSA Free NBP1-77899
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 3 的 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies