NPLOC4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82166

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NPLOC4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPLOC4 Antibody - BSA Free

Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis using Anti-NPLOC4 antibody NBP1-82166 (A) shows similar pattern to independent antibody NBP1-82165 (B).
Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody [NBP1-82166]

Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody [NBP1-82166]

Immunocytochemistry/Immunofluorescence: NPLOC4 Antibody [NBP1-82166] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in human cell line A-549.
Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166]

Western Blot: NPLOC4 Antibody [NBP1-82166] - Whole cell lysate of PC12 using antibody against nuclear protein NPLOC4. Samples were run as triplicates. Image from verified customer review.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166]

Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.

Applications for NPLOC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-82166 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPLOC4

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope

Alternate Names

FLJ20657, FLJ23742, KIAA1499nuclear protein localization protein 4 homolog, NPL4Protein NPL4, nuclear protein localization 4 homolog (S. cerevisiae)

Gene Symbol

NPLOC4

Additional NPLOC4 Products

Product Documents for NPLOC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NPLOC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NPLOC4 Antibody - BSA Free

Customer Reviews for NPLOC4 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • NPLOC4 Antibody
    Name: Luiz Alexandre Magno
    Application: Western Blot
    Sample Tested: PC12 cell lysate
    Species: Rat
    Verified Customer | Posted 10/17/2018
    Whole cell lysate of PC12 using antibody against nuclear protein NPLOC4. Samples were run as triplicates.
    NPLOC4 Antibody - BSA Free NBP1-82166

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