NuMA Antibody

Novus Biologicals | Catalog # NB100-74636

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NuMA Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 900 and 950 of human nuclear mitotic apparatus protein 1 (NP_006176.2).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

238.26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NuMA Antibody

Western Blot: NuMA Antibody [NB100-74636]

Western Blot: NuMA Antibody [NB100-74636]

Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.
Immunohistochemistry: NuMA Antibody [NB100-74636]

Immunohistochemistry: NuMA Antibody [NB100-74636]

Immunohistochemistry: NuMA Antibody [NB100-74636] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUMA used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated

Applications for NuMA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NuMA

The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.

Long Name

Nuclear Mitotic Apparatus Protein 1

Alternate Names

NMP-22, NUMA1, SP-H Antigen

Entrez Gene IDs

4926 (Human)

Gene Symbol

NUMA1

UniProt

Q14980 (Human)

Additional NuMA Products

Product Documents for NuMA Antibody

Certificate of Analysis

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Product Specific Notices for NuMA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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