ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52367

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N497/10

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant protein comprising amino acids aa 161-201 and 343-396 (extracellular and cytoplasmic domains) of mouse ODZ3 (Uniprot#: Q9WTS6) connected by a Gly8 linker, produced recombinantly in E.Coli.

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

303 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N497/10 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N497/10 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Scientific Data Images for ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free [NBP3-52367]

Immunohistochemistry-Paraffin: ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free [NBP3-52367]

Representative image of formalin fixed paraffin embedded section of rat cerebellum section stained with NBP3-52366 (the BSA Free version of this antibody) (brown) and counterstained with hematoxylin (blue). Magnification: 200X
Western Blot: ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free [NBP3-52367]

Western Blot: ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free [NBP3-52367]

Western blotting of rat brain membrane with ODZ3 mouse monoclonal antibody (A) and with NBP3-52366 (the BSA Free version of this antibody) (B) recognizing endogenous ODZ3 at ~300 kDa with similar sensitivity. The mouse monoclonal antibody and chicken monoclonal antibody were detected with anti-mouse-HRP and anti-chicken-HRP, respectively.

Applications for ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ODZ3

ODZ3 may function as a cellular signal transducer

Alternate Names

FLJ10474, FLJ10886, KIAA1455odd Oz/Ten-m homolog 3, odz, odd Oz/ten-m homolog 3 (Drosophila), ODZ3-like protein, Protein Odd Oz/ten-m homolog 3, Ten-3, tenascin-M3, teneurin-3, Ten-M3, TNM3

Gene Symbol

TENM3

Additional ODZ3 Products

Product Documents for ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ODZ3 Antibody (N497/10) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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