Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

Novus Biologicals | Catalog # NBP2-59038

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Cynomolgus Monkey

Cited:

Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4045

Format

BSA Free
View all Ornithine Carbamoyltransferase/OTC Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P00480, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Use in Cynomolgus Monkey reported in scientific literature (PMID:35211641). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining in human liver and tonsil tissues. Corresponding OTC RNA-seq data are presented for the same tissues.
Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunocytochemistry/Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of RH-30 cells using the Anti-OTC monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Applications for Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ornithine Carbamoyltransferase/OTC

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Alternate Names

OCTD, Ornithine Transcarbamylase, OTC, OTCase

Gene Symbol

OTC

Additional Ornithine Carbamoyltransferase/OTC Products

Product Documents for Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Ornithine Carbamoyltransferase Antibody (CL4045) - BSA Free

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Protocols

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FAQs

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