PADI2 Antibody (4D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00011240-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Sandwich ELISA

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D4

Format

Azide and BSA Free
View all PADI2 Primary Antibodies »

Product Specifications

Immunogen

PADI2 (NP_003008, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWVEVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDEEGSIPIDQ

Specificity

PADI2 (4D4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PADI2 Antibody (4D4) - Azide and BSA Free

Sandwich ELISA: PADI2 Antibody (4D4) [H00011240-M01] - Detection limit for recombinant GST tagged PADI2 is approximately 0.3ng/ml as a capture antibody.

Applications for PADI2 Antibody (4D4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Immunohistochemistry was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PADI2

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.

Long Name

Peptidyl Arginine Deiminase type II

Alternate Names

PAD-H19, PAD2, PDI2, Peptidylarginine Deiminase II

Entrez Gene IDs

11240 (Human)

Gene Symbol

PADI2

OMIM

607935 (Human)

UniProt

NP_031391.2 (Human)

Additional PADI2 Products

Product Documents for PADI2 Antibody (4D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PADI2 Antibody (4D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for PADI2 Antibody (4D4) - Azide and BSA Free

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Protocols

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FAQs

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