Parkin Antibody (PRK8)

Novus Biologicals | Catalog # NBP2-29838

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PRK8
View all Parkin Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human Parkin. Epitope has been mapped to the second ring domain (aa 399-465).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Parkin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Relevant Entrez Gene Numbers: NM_004562

Scientific Data Images for Parkin Antibody (PRK8)

Western Blot: Parkin Antibody (PRK8) [NBP2-29838]

Western Blot: Parkin Antibody (PRK8) [NBP2-29838]

Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).
Parkin Antibody (PRK8)

Western Blot: Parkin Antibody (PRK8) [NBP2-29838] -

Immunoblotting analysis of mitophagy proteins. (A) Representative immunoblots for control HT22 cells and mTau-HT22 cells with or without Rlip overexpression and silencing. (B) Represents quantitative densitometry analysis of mitophagy proteins. Pink 1 and Parkin levels were significantly decreased in Mutant Tau when compared with the control. Increased Pink 1 and Parkin levels were observed in Rlip-cDNA with control cells. Decreased Pink 1 and Parkin levels were observed in Tau + Rlip (siRNA) when compared with control HT22 cells. However, significantly increased Pink and Parkin levels were observed in mutant Tau + Rlip-cDNA when compared with the control. (* p = 0.05, ** p = 0.005, *** p = 0.001, and **** p = 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37371116), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Parkin Antibody (PRK8)

Western Blot: Parkin Antibody (PRK8) [NBP2-29838] -

Immunoblotting analysis of mitochondrial mitophagy and synaptic proteins. (A) Representative immunoblots for untreated and hAbKI mice and treated hAbKI Urolithin A and EGCG. (B) Quantitative-densitometry analysis of PINK1, Parkin, synaptophysin and PSD95, which shows significant reduction in the hAbKI mice compared to the treated hAbKI mice Urolithin A and EGCG. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36078067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Parkin Antibody (PRK8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 1 using NBP2-29838 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites, containing 30% glycerol.

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Parkin

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Gene Symbol

PRKN

UniProt

O60260 (Human)

Additional Parkin Products

Product Documents for Parkin Antibody (PRK8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Parkin Antibody (PRK8)

Relevant Entrez Gene Numbers: NM_004562

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Parkin Antibody (PRK8)

Customer Reviews for Parkin Antibody (PRK8) (1)

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  • Parkin Antibody (PRK8)
    Name: Marcel Alavi
    Application: Western Blot
    Sample Tested: Mouse Neuro2A whole cell lysate
    Species: Mouse
    Verified Customer | Posted 01/22/2021
    35 µg N2A whole cell lysate treated wth 7 different mitochondrial stressors, which should result in parkin stabilization according to the literature. The expected size would be around 55 kDa, which is the fifth band from the top.
    Neuro2A cells treated witrh different mitochondrial stressors, which should result in parkin stabilization. However I wasn't able to detect parkin, which should run about the same size as the 5th band from the top (55 kDa.) I repeated the experiment and separated again 35 µg whole cell lysate by a 12% PAGE. I labeled the membrane with parkin 1:400 in 5% dry milk powder in TBST. But again nothing. Happy to share that blot as well by email.
    Parkin Antibody (PRK8) NBP2-29838

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