PARP10 Antibody - BSA Free

Western Blot: PARP10 Antibody - BSA Free [NB100-2157] -

Functional interaction between ATM and PARP10 expression.(A) Western blot showing overexpression of PARP10 in two independent HeLa PARP10 CRISPRa cell lines. (B) CellTiterGlo cellular proliferation assays showing that knockdown of ATM, using two separate siRNA oligonucleotides, specifically reduces the proliferation of two different PARP10-overexpressing CRISPRa HeLa cell lines compared to control HeLa cells. The average of three experiments is shown (normalized to control siRNA). Error bars represent standard deviations, and asterisks indicate statistical significance (t-test, two-tailed, unpaired). (C) DNA fiber combing assays showing that ATM depletion does not differentially impact replication fork progression in PARP10-overexpressing CRISPRa HeLa cell lines compared to control HeLa cells. Replication tracts labeled by both IdU and CldU, indicating ongoing replication forks, were quantified, and their labeled tract length (IdU+CldU) is presented, with the median values marked on the graph and listed at the top. At least 60 tracts were quantified for each sample. Asterisks indicate statistical significance (Mann-Whitney test). A schematic representation of the assay conditions is shown at the top. (D–F) ATM SIRF experiments showing that PARP10 overexpression in HeLa cells increases HU-induced ATM binding to nascent DNA. HeLa cells were treated with 4 mM HU for 3 hours. ATM depletion was used as control, to demonstrate the specificity of the SIRF signal. Representative micrographs (D) and quantifications (E, F) are shown. Bars indicate the mean values, error bars represent standard errors, and asterisks indicate statistical significance (t-test, two-tailed, unpaired). Schematic representations of the assay conditions are shown at the top. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36187556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.