PAWR / PAR4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87338

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAWR / PAR4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAWR / PAR4 Antibody - BSA Free

Western Blot: PAWR / PAR4 Antibody [NBP1-87338]

Western Blot: PAWR / PAR4 Antibody [NBP1-87338]

Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PAWR / PAR4 Antibody [NBP1-87338]

Immunocytochemistry/ Immunofluorescence: PAWR / PAR4 Antibody [NBP1-87338]

Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cell line SiHa shows localization to plasma membrane & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human skeletal muscle shows no positivity as expected.
Western Blot: PAWR / PAR4 Antibody [NBP1-87338]

Western Blot: PAWR / PAR4 Antibody [NBP1-87338]

Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.
Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338]

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.

Applications for PAWR / PAR4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAWR / PAR4

The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq]

Alternate Names

Par-4, PAR4prostate apoptosis response protein 4, PRKC apoptosis WT1 regulator protein, PRKC, apoptosis, WT1, regulator, Prostate apoptosis response 4 protein, prostate apoptosis response protein PAR-4, transcriptional repressor PAR4, WT1-interacting protein

Entrez Gene IDs

5074 (Human)

Gene Symbol

PAWR

UniProt

Q96IZ0 (Human)

Additional PAWR / PAR4 Products

Product Documents for PAWR / PAR4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAWR / PAR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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