PBEF/Visfatin/NAMPT Antibody - BSA Free

Western Blot: PBEF/Visfatin/NAMPT Antibody - BSA Free [NB100-594] -

The Nampt inhibitor, FK866, decreased NAD+ contents and lysosomal functionTime course of intracellular NAD+ or NADH content after addition of the Nampt inhibitor, FK866 (n = 3) in WT MEF cells. The right panel shows what FK866 inhibits in the NAD synthesis pathway.Lysosomal activity was measured by DQ‐BSA in WT and p32KO MEFs. Error bars are presented as mean +/- SEM of three independent experiments. Statistical significance was assessed by Student’s t‐test, **P < 0.002.To monitor autophagosomes and autolysosomes, DAPRed and DALGreen were used. DALGreen fluorescence is enhanced at an acidic pH and is suitable for monitoring the autophagy degradation stage, also known as the autolysosome stage. In contrast, DAPRed has a pH‐independent fluorescence profile and remains fluorescent with almost constant intensity throughout the process of autophagy.Western blot analysis of Lamp2, p62, Nampt, and LC3. Treatment of WT MEFs with FK866 increased the expression of Lamp2, p62, and LC3. When bafilomycin A or FK866 plus NMN were added, the expression of these proteins did not change. One representative experiment out of three shown. Quantification is shown on the right side. The values are mean +/- SD of three independent experiments. Statistical significance was assessed by Student’s t‐test, *P < 0.05, **P < 0.01, ***P < 0.005 (n = 4 per group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33528041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.