PCCB Antibody - BSA Free

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human skin shows only weak cytoplasmic positivity in a subset of epidermal cells.

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human salivary gland.

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human tonsil.

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886]

Immunohistochemistry-Paraffin: PCCB Antibody [NBP1-85886] - Staining of human liver using Anti-PCCB antibody NBP1-85886.

Simple Western: PCCB Antibody [NBP1-85886]

Simple Western: PCCB Antibody [NBP1-85886] - Simple Western lane view shows a specific band for PCCB in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: PCCB Antibody [NBP1-85886]

Simple Western: PCCB Antibody [NBP1-85886] - Electropherogram image(s) of corresponding Simple Western lane view. PCCB antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Immunohistochemistry-Paraffin: PCCB Antibody - BSA Free [NBP1-85886]

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: PCCB Antibody - BSA Free [NBP1-85886]

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Western Blot: PCCB Antibody - BSA Free [NBP1-85886]

Analysis using Anti-PCCB antibody NBP1-85886 (A) shows similar pattern to independent antibody NBP1-85887 (B).

Western Blot: PCCB Antibody - BSA Free [NBP1-85886]

Analysis in human cell line HepG2.

Immunocytochemistry/ Immunofluorescence: PCCB Antibody - BSA Free [NBP1-85886] -

Dual mRNAs encode functional PCC with proper mitochondrial localization in patient-derived fibroblasts and human Hep3B cells.a–c Comparison of co-transfection of hPCCA+hPCCB (dual) mRNAs with single transfection of hPCCA or hPCCB mRNA alone. Human fibroblasts isolated from a PCCA-deficient PA patient and a PCCB-deficient PA patient (n = 2 per condition) were transfected with 0.5 or 1 ug of the total dual mRNAs (1:1 molar ratio), hPCCA mRNA alone, or hPCCB mRNA alone, or 1 μg of eGFP control mRNA for 24 h. Cells were lysed, and mitochondrial matrix fractions were collected to assess PCC enzymatic activity by a radiometric assay (a), and protein levels of PCCA (b) and PCCB (c) by capillary electrophoresis. d Identification of the optimal molar ratio of hPCCA mRNA:hPCCB mRNA. Patient fibroblasts were transfected with 0.67 nM (1 ug) of total dual mRNAs across a range of molar ratios of hPCCA mRNA:hPCCB mRNA or with the same molar concentration of eGFP mRNA (n = 4 per condition) for 24 h. PCC activity in the cellular mitochondrial matrix fractions was measured. Data are shown as mean +/- SEM. e, f Subcellular localization of dual mRNAs-encoded PCC subunits in PCCA-deficient patient fibroblasts and human liver-derived Hep3B cells. Cells were transfected with 25 ng of dual mRNAs or luciferase (Luc) control mRNA for 24 h, and were stained for PCCA, PCCB, and TOM20, a mitochondrial marker. Scale bars are 20 um. Representative images are shown from n = 24 replicates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33087718), licensed under a CC-BY license. Not internally tested by Novus Biologicals.