PCM1 Antibody (CL0206)

Novus Biologicals | Catalog # NBP3-52444

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0206
View all PCM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q15154, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCM1 Antibody (CL0206)

PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0259) [NBP3-52446]

Staining of human fallopian tube shows moderate to strong positivity in the apical cytoplasm of glandular cells.
PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Immunohistochemistry-Paraffin: HMG-CoA Reductase/HMGCR Antibody (CL0260) [NBP3-52447]

Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells as expected.
PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP3-52443]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP3-52443]

Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP3-52448]

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP3-52448]

Analysis in human testis and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.
PCM1 Antibody (CL0206) Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Immunohistochemistry-Paraffin: OTP Antibody (CL11225) [NBP3-52439]

Analysis in human cell line Jurkat.

Applications for PCM1 Antibody (CL0206)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCM1

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Alternate Names

hPCM-1, pericentriolar material 1, pericentriolar material 1 protein, PTC4PCM-1

Gene Symbol

PCM1

Additional PCM1 Products

Product Documents for PCM1 Antibody (CL0206)

Certificate of Analysis

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Product Specific Notices for PCM1 Antibody (CL0206)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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