PHF6 Antibody

Novus Biologicals | Catalog # NB100-68262

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Frozen, Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PHF6 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 315 and 365 of human PHD finger protein 6 (NP_115834.1).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30403997).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41.29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHF6 Antibody

Western Blot: PHF6 Antibody [NB100-68262]

Western Blot: PHF6 Antibody [NB100-68262]

Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.
Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262]

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262]

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: PHF6 Antibody [NB100-68262]

Western Blot: PHF6 Antibody [NB100-68262]

Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.

Applications for PHF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30551478). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32187544). PHF6 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-68262 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHF6

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

BFLS, BORJ, Borjeson-Forssman-Lehmann syndrome, KIAA1823, MGC14797, PHD finger protein 6, PHD-like zinc finger protein

Entrez Gene IDs

84295 (Human)

Gene Symbol

PHF6

UniProt

Q8IWS0 (Human)

Additional PHF6 Products

Product Documents for PHF6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PHF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PHF6 Antibody (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T whole cell lysate prepared with Cell Signal Lysis buffer
    Species: Human
    Verified Customer | Posted 12/05/2013
    PHF6 Antibody NB100-68262

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PHF6 Antibody

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  • Q: Could you please check whether the recombinant protein is conjugated with reactive immunogens as well (like KLH) ?

    A: Unfortunately, those details are part of our production process and we are not able to disclose the information.

  • Q: I read on the webpage that the C terminal is raised by human protein 315-365 amino acid. I am wondering which tag did you guys use to make the recombinant protein as the antigen?

    A: Unfortunately, those details are part of our production process and we are not able to disclose the information.

  • Q: Could you please check whether the recombinant protein is conjugated with reactive immunogens as well (like KLH) ?

    A: Unfortunately, those details are part of our production process and we are not able to disclose the information.

  • Q: I read on the webpage that the C terminal is raised by human protein 315-365 amino acid. I am wondering which tag did you guys use to make the recombinant protein as the antigen?

    A: Unfortunately, those details are part of our production process and we are not able to disclose the information.

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