PIEZO1 Antibody (2-10)

Novus Biologicals | Catalog # NBP2-75617

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Electron Microscopy

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2-10
View all PIEZO1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human PIEZO1 aa 1275-1540 / 2521. (SwissProt: Q92508 Human)

Reactivity Notes

Porcine reactivity reported from a verified customer review. Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Endoplasmic reticulum membrane. Cell membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

287 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIEZO1 Antibody (2-10)

Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617]

Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617]

Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617] - Analysis of FAM38A/PIEZO1 on recombinant protein with Mouse anti-FAM38A/PIEZO1 antibody at 1/500 dilution. Lysates/proteins at 50 ng/Lane.Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617] - Analysis of Siha cells labeling FAM38A/PIEZO1 with Mouse anti-PIEZO1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-PIEZO1 antibody at 1/100 dilution in 2% BSA overnight at 4. Goat Anti-Mouse IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617] - Analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A/PIEZO1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617]

Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617]

Western Blot: PIEZO1 Antibody (2-10) [NBP2-75617] - Porcine endocardial endothelium lysate. Control, mock transfected and Piezo1 siRNA transfected with alpha-actinin as loading control. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617] - ICC staining Protein PIEZO (green) in A431 cells. Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody (2-10) [NBP2-75617] - Analysis of Hela cells labeling FAM38A/PIEZO1 with Mouse anti-PIEZO1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody at 1/100 dilution in 2% BSA overnight at 4. Goat Anti-Mouse IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617] - Analysis of paraffin-embedded mouse brain tissue using anti-Protein FAM38A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617] - Rat brain tissue with Mouse anti-FAM38A/PIEZO1 antibody at 1/600 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617]

Immunohistochemistry-Paraffin: PIEZO1 Antibody (2-10) [NBP2-75617] - Rat hippocampus tissue with Mouse anti-FAM38A/PIEZO1 antibody at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for PIEZO1 Antibody (2-10)

Application
Recommended Usage

Electron Microscopy

Reported in scientific literature (PMID:34489534)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:200-1:600

Simple Western

1:50

Western Blot

1:500-1:2000
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 1 review rated 5 using NBP2-75617 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIEZO1

PIEZO1 (piezo-type mechanosensitive ion channel component 1) plays a crucial role in epithelial cell adhesion. PIEZO1 belongs to the PIEZO family of transmembrane proteins. Mutations in Piezo1 have been tied to Xerocytosis. PIEZO1 has been studied in relation to neuronitis, Alzheimer's disease and Parkinson's disease.

Long Name

Piezo-type mechanosensitive ion channel component 1

Alternate Names

FAM38A, KIAA0233, Mib, Protein FAM38A

Gene Symbol

PIEZO1

Additional PIEZO1 Products

Product Documents for PIEZO1 Antibody (2-10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PIEZO1 Antibody (2-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIEZO1 Antibody (2-10)

Customer Reviews for PIEZO1 Antibody (2-10) (1)

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Customer Images

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  • PIEZO1 Antibody (2-10)
    Name: Charles Cox
    Application: Western Blot
    Sample Tested: Endothelial
    Species: Pig
    Verified Customer | Posted 07/29/2021
    Lysate from pig endocardial endothelium. Control mock transfected and Piezo1 siRNA transfected with alpha-actinin as loading control.
    This was done using 1;1000 NBP2-75617 with secondary anti-mouse IR680 and viewed using the licor odyssey system.
    PIEZO1 Antibody (2-10) NBP2-75617

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