PIMT Antibody

Name: PIMT Antibody
Brand: Novus Biologicals
SKU: NB100-2595
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-2595

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human PRIP-Interacting Protein with Methyltransferase Domain using the numbering given in entry NP_079107.5 (GeneID 96764).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIMT Antibody

Western Blot: PIMT Antibody [NB100-2595]

Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

Immunohistochemistry: PIMT Antibody [NB100-2595]

Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595]

Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.

Immunoprecipitation: PIMT Antibody [NB100-2595]

Immunoprecipitation: PIMT Antibody [NB100-2595] - Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody NB100-2595 (lot NB100-2595-2) used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot NB100-2595-1) and rabbit anti-PIMT antibody NBP2-44294. For blotting immunoprecipitated PIMT, NB100-2595 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Applications for PIMT Antibody

Application

Recommended Usage

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PIMT

Nuclear receptor coactivators participate in the transcriptional activation of specific genes by nuclear receptors. A new nuclear receptor coactivator-interacting protein, designated PIMT, was identified from a human liver cDNA library by using the coactivator peroxisome proliferator-activated receptor-interacting protein (PRIP) as bait in a yeast 2-hybrid screen. The PIMT cDNA encodes an 852-amino acid protein containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Northern blot analysis demonstrated ubiquitous expression of a 3.2-kb PIMT transcript, with highest expression in heart, skeletal muscle, kidney, liver, and placenta. Immunofluorescence studies showed that the PIMT and PRIP proteins are colocalized in the nucleus. PIMT binds 5-adenosyl-L-methionine, the methyl donor for the methyltransfer reaction, and it also binds RNA, suggesting that it is an RNA methyltransferase.

Alternate Names

Cap-specific guanine-N2 methyltransferase, CLL-associated antigen KW-2, DKFZp762A163, EC 2.1.1, EC 2.1.1.-, HCA137, Hepatocellular carcinoma-associated antigen 137, NCOA6IPSEREX-defined, nuclear receptor coactivator 6 interacting protein, Nuclear receptor coactivator 6-interacting protein, PIMTFLJ22995, PIPMT, PRIP-interacting protein PIPMT, PRIP-interacting protein with methyltransferase domain, PRIP-interacting protein with methyltransferase motif, trimethylguanosine synthase, trimethylguanosine synthase 1, trimethylguanosine synthase homolog, trimethylguanosine synthase homolog (S. cerevisiae)

Entrez Gene IDs

96764 (Human)

Gene Symbol

TGS1

UniProt

Q96RS0 (Human)

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Product Documents for PIMT Antibody

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Product Specific Notices for PIMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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