PIP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83386

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Proximity Ligation Assay

Cited:

Western Blot, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PIP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FDIPKSVRPNDEVTAVLAVQTELKECMVVKTYLISSIPLQGAFNYKYTACLCDDNPKTFYWDFYTNRTVQIAAVVDVIRELGICPDDAAVIPIKNNRFYTIEILKVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIP Antibody - BSA Free

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining in human salivary gland and skeletal muscle tissues.. Corresponding PIP RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386]

Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human duodenum shows no positivity in glandular cells as expected.
PIP Antibody - BSA Free Western Blot: PIP Antibody - BSA Free [NBP1-83386]

Western Blot: PIP Antibody - BSA Free [NBP1-83386]

Analysis in human cell line TD47D.

Applications for PIP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Proximity Ligation Assay

Reported in scientific literature (PMID:34440877)

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIP

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Alternate Names

GCDFP15prolactin-inducible protein, GCDFP-15SABP, GPIP4gp17, Gross cystic disease fluid protein 15, prolactin-induced proteinSecretory actin-binding protein

Gene Symbol

PIP

Additional PIP Products

Product Documents for PIP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIP Antibody - BSA Free

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Protocols

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FAQs

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