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Pit1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92273

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (95%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pit1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273]

Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273]

Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human liver, pancreas, pituitary gland and tonsil using Anti-POU1F1 antibody NBP1-92273 (A) shows similar protein distribution across tissues to independent antibody NBP2-55357 (B).
Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273]

Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273]

Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human pituitary gland shows moderate to strong nuclear positivity in neuroendocrine cells in the anterior lobe.
Pit1 Antibody - BSA Free Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Pit1 Antibody - BSA Free Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Pit1 Antibody - BSA Free Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Immunohistochemistry: Pit1 Antibody - BSA Free [NBP1-92273]

Staining of human liver shows no positivity in hepatocytes as expected.

Applications for Pit1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC-P, Retrieval method: HIER pH6

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pit1

The Pit1 gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combin

Alternate Names

CPHD1, GHF1, GHF-1pituitary-specific positive transcription factor 1, Growth hormone factor 1, PIT-1, PIT1pituitary-specific transcription factor 1, POU class 1 homeobox 1, POU domain class 1, transcription factor 1, POU domain, class 1, transcription factor 1, POU1F1a

Gene Symbol

POU1F1

Additional Pit1 Products

Product Documents for Pit1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pit1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pit1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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