PKR Antibody (1D11) - Azide and BSA Free

Novus Biologicals | Catalog # H00005610-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D11

Format

Azide and BSA Free
View all PKR Primary Antibodies »

Product Specifications

Immunogen

EIF2AK2 (NP_002750, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGN

Specificity

EIF2AK2 (1D11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKR Antibody (1D11) - Azide and BSA Free

Western Blot: PKR Antibody (1D11) [H00005610-M02]

Western Blot: PKR Antibody (1D11) [H00005610-M02]

Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PKR Antibody (1D11) [H00005610-M02]

Immunocytochemistry/ Immunofluorescence: PKR Antibody (1D11) [H00005610-M02]

Immunocytochemistry/Immunofluorescence: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on HeLa cell. Antibody concentration 30 ug/ml.
Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02]

Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02]

Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml.
Western Blot: PKR Antibody (1D11) [H00005610-M02]

Western Blot: PKR Antibody (1D11) [H00005610-M02]

Western Blot: PKR Antibody (1D11) [H00005610-M02] - EIF2AK2 monoclonal antibody (M02), clone 1D11 Analysis of EIF2AK2 expression in K-562.
ELISA: PKR Antibody (1D11) [H00005610-M02]

ELISA: PKR Antibody (1D11) [H00005610-M02]

ELISA: PKR Antibody (1D11) [H00005610-M02] - Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.

Applications for PKR Antibody (1D11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKR

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

Long Name

Protein Kinase R

Alternate Names

EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R

Entrez Gene IDs

5610 (Human)

Gene Symbol

EIF2AK2

OMIM

176871 (Human)

UniProt

NP_002750 (Human)

Additional PKR Products

Product Documents for PKR Antibody (1D11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PKR Antibody (1D11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for PKR Antibody (1D11) - Azide and BSA Free

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Protocols

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FAQs

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