PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

Name: PLC-gamma 1 Antibody (2A2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005335-M01
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005335-M01

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 2A2

Format

Azide and BSA Free

View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

PLCG1 (NP_002651, 1192 a.a. ~ 1291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Localization

Cytoplasmic

Specificity

PLCG1 - phospholipase C, gamma 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

Western Blot: PLC-gamma 1 Antibody (2A2) [H00005335-M01]

Western Blot: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - PLCG1 monoclonal antibody (M01), clone 2A2 Analysis of PLCG1 expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: PLC-gamma 1 Antibody (2A2) [H00005335-M01]

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of monoclonal antibody to PLCG1 on HeLa cell. Antibody concentration 40 ug/ml.

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01]

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PTK2 and PLCG1. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01]

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01]

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Detection limit for recombinant GST tagged PLCG1 is approximately 0.1ng/ml as a capture antibody.

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Applications for PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Entrez Gene IDs

5335 (Human)

Gene Symbol

PLCG1

OMIM

172420 (Human)

UniProt

NP_002651 (Human)

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.