PMEL17/SILV Antibody (MSVA-617R)

Novus Biologicals | Catalog # NBP3-57210

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-617R
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Product Specifications

Immunogen

Recombinant fragment of human PMEL protein Swissprot P40967

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin..

Negative Control
Negitve control:Colon: PMEL immunostaining should be completely absent in all cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PMEL17/SILV Antibody (MSVA-617R)

PMEL17/SILV Antibody (MSVA-617R)

Immunohistochemistry-Paraffin:PMEL17/SILV Antibody (MSVA-617R)NBP3-57210

Strong cytoplasmic PMEL immunostaining in all cells of a malignant melanoma of the skin.
PMEL17/SILV Antibody (MSVA-617R)

Immunohistochemistry-Paraffin:PMEL17/SILV Antibody (MSVA-617R)NBP3-57210

Rectum mucosa 1 1
PMEL17/SILV Antibody (MSVA-617R)

Immunohistochemistry-Paraffin:PMEL17/SILV Antibody (MSVA-617R)NBP3-57210

Strong PMEL immunostaining in melanocytes of the skin.
PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Antibody (MSVA-617R) PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

Applications for PMEL17/SILV Antibody (MSVA-617R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (MSVA-617R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PMEL17/SILV Antibody (MSVA-617R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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