PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57297

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-617R

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragment of human PMEL protein Swissprot P40967

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin.

Negative Control
Negitve control:Colon: PMEL immunostaining should be completely absent in all cells.

Scientific Data Images for PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Cancer Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free PMEL17/SILV Normal Tissue Gallery

PMEL17/SILV Normal Tissue Gallery

Applications for PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PMEL17/SILV Antibody (MSVA-617R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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