Podocalyxin Like Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-83348
![Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83348-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD
Reactivity Notes
Reactivity reported in scientific literature (PMID: 22814396)
Marker
Podocytes /Apical Marker, Endothelial Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Podocalyxin Like Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348]
Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, microtubule organizing center & vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0017.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]")
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human liver shows no positivity in hepatocytes as expected.![Western Blot: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Western-Blot-NBP1-83348-img0007.jpg "Western Blot: Podocalyxin Like Antibody [NBP1-83348]")
Western Blot: Podocalyxin Like Antibody [NBP1-83348]
Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney tissue.![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0014.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]")
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0015.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]")
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]](https://resources.rndsystems.com/images/products/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0016.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]")
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348]
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.![Podocalyxin Like Antibody - BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody - BSA Free [NBP1-83348]](https://resources.rndsystems.com/images/products/nbp1-83348_rabbit-polyclonal-podocalyxin-like-antibody-1420251335435.jpg "Immunohistochemistry-Paraffin: Podocalyxin Like Antibody - BSA Free [NBP1-83348]")
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody - BSA Free [NBP1-83348]
Staining of human rectum shows strong membranous positivity in endothelial cells.Applications for Podocalyxin Like Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podocalyxin
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
Additional Podocalyxin Products
Product Documents for Podocalyxin Like Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Podocalyxin Like Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Podocalyxin Like Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Podocalyxin Like Antibody - BSA Free
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Q: Could you please suggest a positive control for this antibody?
A: Podocalyxin is a protein wich is mainly expressed in the glomerular epithelial cells /podocyte in kidney - therefore, you can use a section of human kidney as a positive control for Podocalyxin Antibody NBP1-83348.
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