Polyethylene Glycol Antibody (RM105)
Novus Biologicals | Catalog # NBP2-77399
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
All Species
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM105 expressed in HEK293
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Product Specifications
Immunogen
KLH conjugated Polyethylene Glycol (PEG) with terminal methoxy group
Specificity
This antibody reacts to the methoxy group of Polyethylene glycol (PEG). It is specific for methoxypoly(ethylene glycol).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Polyethylene Glycol Antibody (RM105)
Western Blot: Polyethylene Glycol Antibody (RM105) [NBP2-77399]
Western Blot: Polyethylene Glycol Antibody (RM105) [NBP2-77399] - BSA and PEGylated BSA (mPEG 5 kDa) using 0.1mg/mL of antiPEG clone RM105.Immunohistochemistry: Polyethylene Glycol Antibody (RM105) [NBP2-77399]
Immunohistochemistry: Polyethylene Glycol Antibody (RM105) [NBP2-77399] - Mouse liver and kidney using 0.5mg/mL of anti-PEG Clone RM105. Mouse had been injected with PEG-BSA or BSA for 3 hours before sampling.ELISA: Polyethylene Glycol Antibody (RM105) - Azide and BSA Free [NBP2-77399] -
ELISA: Polyethylene Glycol Antibody (RM105) - Azide and BSA Free [NBP2-77399] - ELISA of three different PEGylated BSAs using Clone RM105, followed by an AP conjugated goat anti-rabbit IgG.Applications for Polyethylene Glycol Antibody (RM105)
Application
Recommended Usage
ELISA
0.01 ug/ml-0.3 ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Western Blot
0.05 ug/ml - 1 ug/ml
Reviewed Applications
Read 1 review rated 5 using NBP2-77399 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Polyethylene Glycol
Alternate Names
1,2-Dihydroxyethane, 1,2-ethanediol, 107-21-1, 2-hydroxyethanol, Ethane-1,2-diol, glycol, Glycol alcohol, monoethylene glycol
Additional Polyethylene Glycol Products
Product Documents for Polyethylene Glycol Antibody (RM105)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Polyethylene Glycol Antibody (RM105)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Polyethylene Glycol Antibody (RM105) (1)
5 out of 5
1 Customer Rating
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Application: Immunohistochemistry-ParaffinSample Tested: Kidney tissueSpecies: MouseVerified Customer | Posted 08/17/2022mouse kidney
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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