PQBP1 Antibody

Novus Biologicals | Catalog # NBP1-47271

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 215 and 265 of human Polyglutamine Binding Protein 1 using the numbering given in entry NP_005701.1 (GeneID 10084).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PQBP1 Antibody

Western Blot: PQBP1 Antibody [NBP1-47271]

Western Blot: PQBP1 Antibody [NBP1-47271]

Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Immunohistochemistry: PQBP1 Antibody [NBP1-47271]

Immunohistochemistry: PQBP1 Antibody [NBP1-47271]

Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for PQBP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PQBP1

PQBP1 is a nuclear polyglutamine-binding protein that contains a WW domain (Waragai et al., 1999 [PubMed 10332029]).[supplied by OMIM]

Alternate Names

38 kDa nuclear protein containing a WW domain, MRX55, MRXS3, MRXS8, Npw38, NPW38mental retardation, X-linked 55, nuclear protein containing WW domain 38 kD, polyglutamine binding protein 1, Polyglutamine tract-binding protein 1, polyglutamine-binding protein 1, PQBP-1, RENS1, SHS, Sutherland-Haan X-linked mental retardation syndrome

Entrez Gene IDs

10084 (Human)

Gene Symbol

PQBP1

Additional PQBP1 Products

Product Documents for PQBP1 Antibody

Certificate of Analysis

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Product Specific Notices for PQBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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