PRKCSH Antibody

Novus Biologicals | Catalog # NBP2-19931

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PRKCSH Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary.

Reactivity Notes

Pig (86%), Bovine (83%).

Localization

Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRKCSH Antibody

Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-19931]

Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-19931]

Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-19931] - Paraffin-embedded U87 xenograft. PRKCSH antibody dilution: 1:500.
Western Blot: PRKCSH Antibody [NBP2-19931]

Western Blot: PRKCSH Antibody [NBP2-19931]

Western Blot: PRKCSH Antibody [NBP2-19931] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7. 5% SDS PAGE gel, diluted at 1:10000.
PRKCSH Antibody

Western Blot: PRKCSH Antibody [NBP2-19931] -

Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody (NBP2-19931) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PRKCSH Antibody

Immunocytochemistry/ Immunofluorescence: PRKCSH Antibody [NBP2-19931] -

PRKCSH antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRKCSH protein stained by PRKCSH antibody (NBP2-19931) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for PRKCSH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRKCSH

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

AGE-R2,80K-H protein, G19P1AGE-binding receptor 2, glucosidase 2 subunit beta, Glucosidase II subunit beta, glucosidase II, beta subunit, hepatocystin, PCLD, PKCSH, PLD1, Protein kinase C substrate 60.1 kDa protein heavy chain, protein kinase C substrate 80K-H, protein kinase C substrate, 80 Kda protein

Gene Symbol

PRKCSH

UniProt

P14314 (Human)

Additional PRKCSH Products

Product Documents for PRKCSH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PRKCSH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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