Proteasome 20S alpha 3 Antibody (24GB1665)

Novus Biologicals | Catalog # NBP3-50615

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1665
View all Proteasome 20S alpha 3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Proteasome 20S alpha 3 (Uniprot: P25788)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S alpha 3 Antibody (24GB1665)

Proteasome 20S alpha 3 Antibody (24GB1665)

Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 3 Antibody (24GB1665)[NBP3-50615] -

Immunocytochemical staining of HepG2 cells with Proteasome 20S alpha 3 antibody (1:1,000). Nuclei were stained blue with DAPI; Proteasome 20S alpha 3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Proteasome 20S alpha 3 Antibody (24GB1665)

Flow Cytometry: Proteasome 20S alpha 3 Antibody (24GB1665)[NBP3-50615] -

Flow cytometric analysis of Proteasome 20S alpha 3expression in HepG2 cells using Proteasome 20S alpha 3 antibody (1:2,000). Green, isotype control; red, PSMA3.
Proteasome 20S alpha 3 Antibody (24GB1665)

Western Blot: Proteasome 20S alpha 3 Antibody (24GB1665)[NBP3-50615] -

Western blotting analysis using Proteasome 20S alpha 3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Proteasome 20S alpha 3 Antibody (24GB1665)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Proteasome subunit alpha type-3

Alternate Names

HC8, PSC8, PSMA3

Gene Symbol

PSMA3

Additional Proteasome 20S alpha 3 Products

Product Documents for Proteasome 20S alpha 3 Antibody (24GB1665)

Certificate of Analysis

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Product Specific Notices for Proteasome 20S alpha 3 Antibody (24GB1665)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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