Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Novus Biologicals | Catalog # NBP1-47935

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6C7
View all Protein Kinase A regulatory subunit I alpha Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase A regulatory subunit I alpha monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of paraffin-embedded carcinoma of bladder tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50).
Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase A regulatory subunit I alpha.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded colon tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded endometrium tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Ovary tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935]

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded prostate tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)[NBP1-47935]

Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)[NBP1-47935]Simple Western� analysis of endogenous protein SERPINA1 from HepG2 lysates (0.2 mg/mL) using SERPINA1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-47935 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Entrez Gene IDs

5573 (Human)

Gene Symbol

PRKAR1A

Additional Protein Kinase A regulatory subunit I alpha Products

Product Documents for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Certificate of Analysis

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Product Specific Notices for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)

Customer Reviews for Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) (1)

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  • Protein Kinase A regulatory subunit I alpha Antibody (6C7)
    Name: Daniel Popoola
    Application: Western Blot
    Sample Tested: Dorsal Hippocampus Brain Tissue
    Species: Rat
    Verified Customer | Posted 02/13/2017
    Samples are dorsal hippocampus samples from Long Evans rats. Ladders (on the right) are 250, 98, 64,50,36, & 30 kDa (from top to bottom). Expected band size is 43kDa
    Diluted 1:2000
    Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) NBP1-47935

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