Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73659

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6C7

Format

Azide and BSA Free
View all Protein Kinase A regulatory subunit I alpha Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)Azide and BSA Free [NBP2-73659]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)Azide and BSA Free [NBP2-73659]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and im
Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded prostate tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)Azide and BSA Free [NBP2-73659]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7)Azide and BSA Free [NBP2-73659]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase A regulatory subunit I alpha monoclonal antibody.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded colon tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded endometrium tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded Ovary tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.
Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659]

Immunohistochemistry: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) - Azide and BSA Free [NBP2-73659] - Staining of paraffin-embedded carcinoma of bladder tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50).

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Gene Symbol

PRKAR1A

Additional Protein Kinase A regulatory subunit I alpha Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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