Protein Phosphatase 1 beta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32618

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (99%), Chicken (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein Phosphatase 1 beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Protein Phosphatase 1 beta. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Phosphatase 1 beta Antibody - BSA Free

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Protein Phosphatase 1 beta Antibody detects Protein Phosphatase 1 beta protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody diluted at 1:3000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Mouse hind brain dilution: 1:500.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Proetin Phosphatase 1 beta Antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Proetin Phosphatase 1 beta stained by Proetin Phosphatase 1 beta Antibody diluted at 1:3000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Protein Phosphatase 1 beta Antibody

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP1-32618] -

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Protein Phosphatase 1 beta antibody detects Protein Phosphatase 1 beta protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:500.
Blue: Hoechst 33342 staining.
Protein Phosphatase 1 beta Antibody

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] -

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Protein Phosphatase 1 beta Antibody

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] -

Western Blot: Protein Phosphatase 1 beta Antibody [NBP1-32618] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Protein Phosphatase 1 beta Antibody - BSA Free

Immunohistochemistry-Frozen: Protein Phosphatase 1 beta Antibody [NBP1-32618] -

Protein Phosphatase 1 beta antibody detects Protein Phosphatase 1 beta Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Protein Phosphatase 1 beta stained by Protein Phosphatase 1 beta antibody (NBP1-32618) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Applications for Protein Phosphatase 1 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protein Phosphatase 1 beta

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Alternate Names

EC 3.1.3.16, EC 3.1.3.53, MGC3672, PP1beta, PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1-beta, protein phosphatase 1-delta, serine/threonine protein phosphatase PP1-beta catalytic subunit, serine/threonine-protein phosphatase PP1-beta catalytic subunit

Gene Symbol

PPP1CB

Additional Protein Phosphatase 1 beta Products

Product Documents for Protein Phosphatase 1 beta Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Protein Phosphatase 1 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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