PrPSc Antibody (1.5D7)

Novus Biologicals | Catalog # NBP3-11229

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Bovine, Hamster, Sheep

Applications

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1.5D7
View all PrPSc Primary Antibodies »

Product Specifications

Immunogen

BoPrP153-165 (GSDYEDRYYRENM)

Specificity

The monoclonal antibody 1.5D7 recognizes the disease associated isoform of the prion protein termed PrPSc.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for PrPSc Antibody (1.5D7)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The monoclonal antibody can be used for the specific identification and characterization of PrPSc in tissue sections by immunohistochemistry and PET-blot. For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PrPSc

Prion diseases are transmissible neurodegenerative disorders which affect a range of mammalian species. In humans they can be inherited and sporadic as well as acquired by exposure to human prions. Prions appear to be composed principally of a conformational isomer of host-encoded prion protein and propagate by recruitment of cellular prion protein (1). The function of the cellular prion protein (PrP) is still poorly understood. It has been proposed that one unprecedented role for PrP is against Bax-mediated neuronal apoptosis. It has been shown that PrP potently inhibits Bax-induced cell death in human primary neurons (2). An impaired synaptic inhibition may be involved in the epileptiform activity seen in Creutzfeldt-Jakob and other neurodegenerative diseases and it is believed that loss of function of PrP may contribute to the early synaptic loss and neuronal degeneration seen in these diseases (3).

Alternate Names

Prion Protein Scrapie

Gene Symbol

PRNP

Additional PrPSc Products

Product Documents for PrPSc Antibody (1.5D7)

Certificate of Analysis

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Product Specific Notices for PrPSc Antibody (1.5D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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