PSRC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88166

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSRC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSRKQNYSSKNENCVEETFEDLLLKYKQIQLELECINKDEKLALSSKEENVQEDPKTLNFEDQTSTDNVSITKDSSKEVAPEEKTQVKTFQAFELKPLRQKLTLPGDKNRLKKVKDGAKPLSL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (84%). Reactivity reported in scientific literature (PMID: 25268596)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSRC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PSRC2 Antibody [NBP1-88166]

Immunocytochemistry/ Immunofluorescence: PSRC2 Antibody [NBP1-88166]

Immunocytochemistry/Immunofluorescence: PSRC2 Antibody [NBP1-88166] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166] - Staining of human cerebral cortex shows moderate positivity in neurons.
Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166] - Saining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166] - Staining of human prostate shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166]

Immunohistochemistry-Paraffin: PSRC2 Antibody [NBP1-88166] - Analysis in human testis and skeletal muscle tissues. Corresponding ZFC3H1 RNA-seq data are presented for the same tissues.

Applications for PSRC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IP reported in scientific literature (PMID: 25268596). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSRC2

CCDC131 (coiled-coil domain containing protein 131) is an uncharacterized proline/serine rich coiled-coil protein that bears 6 HAT repeats- and 5 TPR repeats. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization. HAT (half a TPR) repeats form amphipathic alpha-helices and are similar to TPR repeats which are 34 amino acid repeat motifs which form alpha helical structures.

Alternate Names

coiled-coil domain containing 131, Coiled-coil domain-containing protein 131, DKFZp686A0722, KIAA0546CCDC131, MGC23401, MGC90200, proline/serine-rich coiled-coil 2, Proline/serine-rich coiled-coil protein 2, PSRC2, zinc finger C3H1 domain-containing protein, zinc finger, C3H1-type containing

Entrez Gene IDs

196441 (Human)

Gene Symbol

ZFC3H1

UniProt

O60293 (Human)

Additional PSRC2 Products

Product Documents for PSRC2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PSRC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSRC2 Antibody - BSA Free

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Protocols

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