PTBP1 Antibody (3H8) - Azide and BSA Free

Novus Biologicals | Catalog # H00005725-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Single Cell Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H8

Format

Azide and BSA Free
View all PTBP1 Primary Antibodies »

Product Specifications

Immunogen

PTBP1 (NP_002810, 45 a.a. ~ 144 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQ

Specificity

PTBP1 - polypyrimidine tract binding protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PTBP1 Antibody (3H8) - Azide and BSA Free

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01]

Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01]

Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01]

Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01]

Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 2 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 5 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01]

Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - PTBP1 monoclonal antibody (M01), clone 3H8 Analysis of PTBP1 expression in A-431.
Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01]

Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01]

Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 transfected lysate using anti-PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 MaxPab rabbit polyclonal antibody.
ELISA: PTBP1 Antibody (3H8) [H00005725-M01]

ELISA: PTBP1 Antibody (3H8) [H00005725-M01]

ELISA: PTBP1 Antibody (3H8) [H00005725-M01] - Detection limit for recombinant GST tagged PTBP1 is approximately 0.1ng/ml as a capture antibody.

Applications for PTBP1 Antibody (3H8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Single Cell Western

1:10

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. SCW is validated using U373 cells.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTBP1

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

Heterogeneous nuclear ribonucleoprotein I, heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, HNRNPI, HNRNP-I, HNRPI, MGC10830, MGC8461, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoproteinI), polypyrimidine tract binding protein 1, polypyrimidine tract-binding protein 1, pPTB, PTB-1, PTB2, PTB3, PTB4, PTB57 kDa RNA-binding protein PPTB-1, PTB-T, RNA-binding protein

Entrez Gene IDs

5725 (Human)

Gene Symbol

PTBP1

OMIM

600693 (Human)

UniProt

NP_002810 (Human)

Additional PTBP1 Products

Product Documents for PTBP1 Antibody (3H8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PTBP1 Antibody (3H8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PTBP1 Antibody (3H8) - Azide and BSA Free

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Protocols

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