PTCD2 Antibody

Western Blot: PTCD2 Antibody [NBP2-26176]

Western Blot: PTCD2 Antibody [NBP2-26176] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: PTCD2 Antibody [NBP2-26176]

Immunohistochemistry-Paraffin: PTCD2 Antibody [NBP2-26176] - (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: PTCD2 Antibody [NBP2-26176] -

PTCD2 interacts with associated mitochondrial ribosomes. (A) Proteomic data analysis. The diagram represents the protein species identified in the samples of coIP and negative control. Label free quantification (LFQ) intensities for all proteins were calculated with Max Quant software. LFQs of the proteins in coIP samples are placed along the vertical axis; LFQs of the same proteins in negative control samples are placed along the horizontal axis. Blue dots show each individual protein. Yellow dots indicate mitochondrial ribosomal proteins. Red line shows the position of the points where proteins are equally represented in coIP and negative control. Green line is a threshold line showing the positions of the points where proteins are 1.8 times enriched in coIP relative to control. (B) Western blot analysis of fractionated mitochondrial ribosomes. Clarified HeLa cell lysate was applied to 10–30% linear sucrose gradient and ultracentrifuged. The gradient was fractionated from top to bottom in 21 fractions and proteins from each fraction were precipitated and applied to Western blot with the indicated antibodies. LC—loading control (0.1% from total lysate volume), SSU—mitoribosome small subunit, LSU—mitoribosome large subunit, mitoR—associated mitoribosome. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36430722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.