PTPIP51 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-47293
![Immunohistochemistry-Paraffin: PTPIP51 Antibody [NBP1-47293]](https://resources.rndsystems.com/images/products/PTPIP51-Antibody-Immunohistochemistry-Paraffin-NBP1-47293-img0003.jpg "Immunohistochemistry-Paraffin: PTPIP51 Antibody [NBP1-47293]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 300 and 350 of human Protein Tyrosine Phosphatase-interacting Protein 51 (PTPIP51) using the numbering given in entry NP_060615.1 (GeneID 55177).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PTPIP51 Antibody - BSA Free
Immunohistochemistry-Paraffin: PTPIP51 Antibody [NBP1-47293]
Immunohistochemistry-Paraffin: PTPIP51 Antibody [NBP1-47293] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-PTPIP51 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB![Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]](https://resources.rndsystems.com/images/products/PTPIP51-Antibody-Immunoprecipitation-NBP1-47293-img0002.jpg "Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]")
Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]
Immunoprecipitation: PTPIP51 Antibody [NBP1-47293] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPIP51 antibody NBP1-47293 used for IP at 3 ug/mg lysate. PTPIP51 was also immunoprecipitated by rabbit anti-PTPIP51 antibody NBP1-47294, which recognizes a downstream epitope. For blotting immunoprecipitated PTPIP51, NBP1-47294 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]](https://resources.rndsystems.com/images/products/PTPIP51-Antibody-Immunoprecipitation-NBP1-47293-img0004.jpg "Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]")
Immunoprecipitation: PTPIP51 Antibody [NBP1-47293]
Immunoprecipitation: PTPIP51 Antibody [NBP1-47293] - Detection of human PTPIP51 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPIP51 antibody NBP1-47293 used for IP at 3 ug per reaction. PTPIP51 was also immunoprecipitated by rabbit anti-PTPIP51 antibody NBP1-47294. For blotting immunoprecipitated PTPIP51, NBP1-47293 was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.Applications for PTPIP51 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PTPIP51
Alternate Names
Cerebral protein 10, FAM82Cmicrotubule-associated protein, family with sequence similarity 82, member A2, FLJ10579, hRMD-3, Protein FAM82A2, Protein FAM82C, Protein tyrosine phosphatase-interacting protein 51, ptpip51, PTPIP51family with sequence similarity 82, member C, regulator of microtubule dynamics 3, regulator of microtubule dynamics protein 3, RMD3, RMD-3, TCPTP-interacting protein 51
Entrez Gene IDs
55177 (Human)
Gene Symbol
RMDN3
Additional PTPIP51 Products
Product Documents for PTPIP51 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PTPIP51 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PTPIP51 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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