PYK2/FAK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91228

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PYK2/FAK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPM

Reactivity Notes

Mouse (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PYK2/FAK2 Antibody - BSA Free

Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in human cell lines A-431 and HEK293 using anti-PTK2B antibody. Corresponding PTK2B RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by Western blot. Image submitted by a verified customer review.
Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PYK2/FAK2 Antibody - BSA Free Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PYK2/FAK2 Antibody - BSA Free Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]

Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]

Analysis using antibody NBP1-91228 (A) shows similar pattern to independent antibody NBP1-91227 (B).

Applications for PYK2/FAK2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-91228 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PYK2/FAK2

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Long Name

Proline-rich Tyrosine Kinase 2

Alternate Names

CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK

Gene Symbol

PTK2B

Additional PYK2/FAK2 Products

Product Documents for PYK2/FAK2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PYK2/FAK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PYK2/FAK2 Antibody - BSA Free

Customer Reviews for PYK2/FAK2 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • PYK2/FAK2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 09/21/2018
    Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by western blot.
    PYK2/FAK2 Antibody - BSA Free NBP1-91228

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