Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunoblotting, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Pyruvate Dehydrogenase E1-alpha subunit Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide surrounding the phosphorylated serine 293 of the human Pyruvate Dehydrogenase E1-alpha subunit protein. [Swiss-Prot: #P08559]

Modification

p Ser293

Localization

Mitochondrion matrix

Specificity

This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pyruvate Dehydrogenase E1-alpha subunit

Pyruvate dehydrogenase (PDH) complex is localized to mitochondrial matrix, wherein it catalyzes the irreversible oxidative decarboxylation of pyruvate to generate acetyl-CoA, NADH, and CO2. The PDH complex contains three primary enzyme components: pyruvate dehydrogenase (E1 or PDHA- heterotetramer of two alpha- and two beta-subunits), dihydrolipoamide transacetylase (E2 or DLAT), and dihydrolipoamide dehydrogenase (E3 or DLD). The activity of PDH is controlled by specific E1 kinase and phospho-E1-phosphatase enzymes, which respectively inactivate and activate PDH complex by phosphorylation and dephosphorylation of E1alpha-subunit's serine residues. PDK family kinases mediated phosphorylation at Ser-293 blocks the access to active site leading to inactivation of the enzyme and vice versa. E3-binding protein (E3BP) is another component which is required for proper interaction of E2 and E3 components, and the overall complex contains 12 copies of E3, 30 copies of E1, 60 copies of E2, and 12 copies of E3BP. Acetyl-CoA generated gets utilized in TCA (citric acid/Krebs cycle) where it reacts with oxaloacetate to form citrate or it may also be used for biosynthesis of fatty acid or cholesterol. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD), X-linked Leigh syndrome (X-LS) and it has also been implicated in ageing, glucose intolerance, cancer as well as Alzheimer's disease.

Alternate Names

EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Gene Symbol

PDHA1

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Product Documents

Certificate of Analysis

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Product Specific Notices



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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