QARS Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37883

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all QARS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for QARS Antibody - BSA Free

Western Blot: QARS Antibody [NBP2-37883]

Western Blot: QARS Antibody [NBP2-37883]

Western Blot: QARS Antibody [NBP2-37883] - Analysis using Anti-QARS antibody NBP2-37883 (A) shows similar pattern to independent antibody NBP1-89487 (B).
Immunocytochemistry/ Immunofluorescence: QARS Antibody [NBP2-37883]

Immunocytochemistry/ Immunofluorescence: QARS Antibody [NBP2-37883]

Immunocytochemistry/Immunofluorescence: QARS Antibody [NBP2-37883] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human colon.
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human cerebral cortex, colon, liver and testis using Anti-QARS antibody NBP2-37883 (A) shows similar protein distribution across tissues to independent antibody NBP1-89487 (B).
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human testis.
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883]

Immunohistochemistry-Paraffin: QARS Antibody [NBP2-37883] - Staining of human liver.

Applications for QARS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: QARS

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

Alternate Names

EC 6.1.1, EC 6.1.1.18, GlnRS, glutamine tRNA ligase, Glutamine--tRNA ligase, glutamine-tRNA synthetase, glutaminyl-tRNA synthetase, PRO2195

Gene Symbol

QARS1

UniProt

P47897 (Human)

Additional QARS Products

Product Documents for QARS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for QARS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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