RAD18 Antibody (3H7) - Azide and BSA Free

Novus Biologicals | Catalog # H00056852-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3H7

Format

Azide and BSA Free
View all RAD18 Primary Antibodies »

Product Specifications

Immunogen

RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKEIDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQEDNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEV

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26549024). Other species have not been tested.

Specificity

RAD18 - RAD18 homolog (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for RAD18 Antibody (3H7) - Azide and BSA Free

Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01]

Immunocytochemistry/ Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01]

Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.
Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01]

Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01]

Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # H00056852-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.
Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01]

Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7 Analysis of RAD18 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01]

Immunocytochemistry/ Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01]

Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.
ELISA: RAD18 Antibody (3H7) [H00056852-M01]

ELISA: RAD18 Antibody (3H7) [H00056852-M01]

ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.

Applications for RAD18 Antibody (3H7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

25 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1.5 ug/ml

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reviewed Applications

Read 2 reviews rated 3.5 using H00056852-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAD18

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Entrez Gene IDs

56852 (Human)

Gene Symbol

RAD18

OMIM

605256 (Human)

UniProt

NP_064550 (Human)

Additional RAD18 Products

Product Documents for RAD18 Antibody (3H7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAD18 Antibody (3H7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAD18 Antibody (3H7) - Azide and BSA Free

Customer Reviews for RAD18 Antibody (3H7) - Azide and BSA Free (2)

3.5 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
50%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • RAD18 Antibody (3H7)
    Name: Lisa Moreau
    Application: Immunofluorescence
    Sample Tested: Human cells
    Species: Human
    Verified Customer | Posted 09/12/2017
    The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue).
    RAD18 Antibody (3H7) - Azide and BSA Free H00056852-M01
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cell culture, Sample Amount: 50ug
    Species: Human
    Verified Customer | Posted 07/06/2009

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