Rat IL-2 Antibody [Biotin]

R&D Systems | Catalog # BAF502

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL-2 (R&D Systems, Catalog # 502-RL)
Ala21-Gln155
Accession # P17108

Specificity

Detects rat IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2 and recombinant mouse IL-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IL-2 Antibody [Biotin]

Rat IL-2 ELISA Standard Curve

Rat IL-2 ELISA Standard Curve

Recombinant Rat IL‑2 (Catalog # 502-RL) was serially diluted and captured by Goat Anti-Human/Rat IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-502-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Rat IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-502-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat IL-2 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL‑2 (Catalog # 502-RL)

Rat IL-2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Rat IL‑2 Antibody (Catalog # AF-502-NA)
  • Standard: Recombinant Rat IL-2 Protein (Catalog # 502-RL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF502 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2, respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (4 - 6). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain  gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (7). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (8 - 10). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (11, 12).

References

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. McKnight, A. et al. (1989) Immunogenetics 30:145.
  4. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  5. Wang, X. et al. (2005) Science 310:1159.
  6. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  7. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  8. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  9. Laurence, A. et al. (2007) Immunity 26:371.
  10. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  11. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  12. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

P17108

Additional IL-2 Products

Product Documents for Rat IL-2 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat IL-2 Antibody [Biotin]

For research use only

Citations for Rat IL-2 Antibody [Biotin]

Customer Reviews for Rat IL-2 Antibody [Biotin] (2)

4.5 out of 5
2 Customer Ratings
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  • Rat IL-2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Rat
    Verified Customer | Posted 11/08/2017
    This antibody was used as a detection with AF502 as the capture in an ELISA to measure Rat IL-2 in serum samples.
    Rat IL-2 Antibody [Biotin] BAF502
  • Rat IL‑2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/26/2015
    ELISA plates were coated by incubating the diluted capture antibody (anti-rat IL2,R&D systems) overnight at 4C. To detect the plate-bound IL2 from the culture supernatant, the detection antibody (biotin anti-rat IL2) was used at 400ng/mL, followed by avidin-HRP. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: blocking buffer, wash buffer<br />Dilution: 400ng/mL
    Rat IL-2 Antibody [Biotin] BAF502

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