RecA-1 Antibody (HIS52) - BSA Free
Novus Biologicals | Catalog # NB100-64647
![Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) - BSA Free [NB100-64647]](https://resources.rndsystems.com/images/products/RecA-1-Antibody-HIS52-Immunocytochemistry-Immunofluorescence-NB100-64647-img0001.jpg "Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) - BSA Free [NB100-64647]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunofluorescence
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # HIS52
Format
BSA Free
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Product Specifications
Immunogen
Stromal cells from rat lymph node
Reactivity Notes
Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig
Specificity
Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for RecA-1 Antibody (HIS52) - BSA Free
Applications for RecA-1 Antibody (HIS52) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RecA-1
Alternate Names
RecA protein, RecA1, RecA2, recaLP, Recombinase A
Additional RecA-1 Products
Product Documents for RecA-1 Antibody (HIS52) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RecA-1 Antibody (HIS52) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for RecA-1 Antibody (HIS52) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for RecA-1 Antibody (HIS52) - BSA Free
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Q: Did you test this Ab also on human endothelial cells (ECs)? I`m looking for an ab, which recognizes only rat ECs and not human.
A: We have not tested this on human cells so we would not be able to say if it would recognize human or not. I have searched for another RecA-1 antibody for you but currently we only have the one.
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