Recombinant Human BCMA/TNFRSF17 Fc Biotinylated Protein, CF New

Catalog #: BT193 Datasheet / COA / SDS

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BT193-050

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Biotinylated Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BCMA/TNFRSF17 Fc Biotinylated Protein, CF Summary

R&D Systems NS0-derived Recombinant Human BCMA/TNFRSF17 Fc Biotinylated Protein (BT193)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # BT193) binds Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP/CF) with an ED₅₀ of 0.500-6.00 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein

Human BCMA/TNFRSF17 (Met1-Ala54) Accession # Q6PE46	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6PE46

N-terminal Sequence
Analysis

Met 1

Structure / Form

Disulfide-linked homodimer, biotinylated via amines

Predicted Molecular Mass

32 kDa

SDS-PAGE

31-48 kDa, under reducing conditions.

Product Datasheets

BT193

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT193

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Biotinylated Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein (Catalog # BT193) binds Recombinant Human APRIL/TNFSF13 (5860-AP/CF) with an ED₅₀ of 0.500-6.00 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein (Catalog # BT193) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 31-48 kDa and 60-100 kDa, respectively.

Reconstitution Calculator

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17